Construction of High-Quality Camel Immune Antibody Libraries

https://pubmed.ncbi.nlm.nih.gov/29116505/
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Construction of High-Quality Camel Immune Antibody Libraries ... Ema Romão , Vianney Poignavent , Cécile Vincke , Christophe Ritzenthaler , Serge ... Public health information (CDC) Prevention and treatment information (HHS) Access keysNCBI HomepageMyNCBI HomepageMain ContentMain Navigation . 2018;1701:169-187. doi: 10.1007/978-1-4939-7447-4_9. Construction of High-Quality Camel Immune Antibody Libraries Ema Romão  1 , Vianney Poignavent  2 , Cécile Vincke  1 , Christophe Ritzenthaler  2 , Serge Muyldermans  3 , Baptiste Monsion  2 DOI: 10.1007/978-1-4939-7447-4_9 Single-domain antibodies libraries of heavy-chain only immunoglobulins from camelids or shark are enriched for high-affinity antigen-specific binders by a short in vivo immunization. Thus, potent binders are readily retrieved from relatively small-sized libraries of 107-108 individual transformants, mostly after phage display and panning on a purified target. However, the remaining drawback of this strategy arises from the need to generate a dedicated library, for nearly every envisaged target. Therefore, all the procedures that shorten and facilitate the construction of an immune library of best possible quality are definitely a step forward. In this chapter, we provide the protocol to generate a high-quality immune VHH library using the Golden Gate Cloning strategy employing an adapted phage display vector where a lethal ccdB gene has to be substituted by the VHH gene. With this procedure, the construction of the library can be shortened to less than a week starting from bleeding the animal. Our libraries exceed 108 individual transformants and close to 100% of the clones harbor a phage display vector having an insert with the length of a VHH gene. These libraries are also more economic to make than previous standard approaches using classical restriction enzymes and ligations. The quality of the Nanobodies that are retrieved from immune libraries obtained by Golden Gate Cloning is identical to those from immune libraries made according to the classical procedure.



Remote Sensing | Free Full-Text | Geo-Registering Consecutive ...

https://www.mdpi.com/2072-4292/12/12/1928
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Geo-Registering Consecutive Construction Site Recordings Using a Pre-Registered Reference Module . by. Stan Vincke. * and. Maarten Vergauwen. Journals Remote Sensing Volume 12 Issue 12 10.3390/rs12121928 Submit to this Journal Review for this Journal Edit a Special Issue Geo-Registering Consecutive Construction Site Recordings Using a Pre-Registered Reference Module † by Stan Vincke * and Maarten Vergauwen Department of Civil Engineering, Geomatics Research Group, KU Leuven-Faculty of Engineering Technology, 9000 Ghent, Belgium Author to whom correspondence should be addressed. This paper is an extended version of our paper published in the ISPRS archives, presented at the Measurement, Visualisation and Processing in BIM for Design and Construction Management conference in Prague, Czech Republic, doi:10.5194/isprs-archives-XLII-5-W2-85-2019. Received: 30 April 2020 / Revised: 27 May 2020 / Accepted: 8 June 2020 / Published: 15 June 2020 (This article belongs to the Special Issue Image Processing and Analysis: Trends in Registration, Data Fusion, 3D Reconstruction and Change Detection) View Full-Text Download PDF Browse Figures Citation Export Keywords: registration; photogrammetry; construction; progress monitoring; ground control This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited Remote Sens., EISSN 2072-4292, Published by MDPI Disclaimer RSS Content Alert Subscribe to receive issue release notifications and newsletters from MDPI journals © 1996-2021 MDPI (Basel, Switzerland) unless otherwise stated Disclaimer Terms and Conditions Privacy Policy



Eric Vincke

www.goslingczubak.com/team-members/eric-vincke.html
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Eric Vincke serves as a Project Scientist assisting multiple oil and gas and ... He oversees landfill construction, including capping, liner replacement and ... Access denied due to malicious activity




Stan Vincke – Geomatics

https://iiw.kuleuven.be/onderzoek/geomatics/people/00117444
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Ing. Stan Vincke completed his master's degree (Construction Engineering Technology – Surveying, KU Leuven) in 2017. In January 2018 he started his PhD, ... Ing. Stan Vincke completed his master’s degree (Construction Engineering Technology – Surveying, KU Leuven) in 2017. In January 2018 he started his PhD, funded by the acquired FWO SB grant, titled “Construction site monitoring using photogrammetry”. Furthermore he is responsible for the course 3D modelling in the master’s year in surveying. His research interests include photogrammetry, both terrestrial and aerial, construction site monitoring, Scan to BIM and automation in modelling. SORT newest first |author |title |popularity TYPE all | articles | presentations | conferences | chapters | other | reports | phd theses | book reviews | books | outreach | edited books journal-article Bassier, maarten; Vincke, stan; De Winter, heinder; Vergauwen, Maarten; 2020. Drift Invariant Metric Quality Control of Construction Sites Using BIM and Point Cloud Data. Isprs International Journal Of Geo-Information; 2020; Vol. 9; iss. 9; pp. - keyboard_arrow_down journal-article Vincke, stan; Vergauwen, Maarten; 2020. Geo-Registering Consecutive Construction Site Recordings Using a Pre-Registered Reference Module. Remote Sensing; 2020; Vol. 12; iss. 12; pp. - keyboard_arrow_down conference Vincke, Stan; Vergauwen, maarten; 2019. Geo-Registering Consecutive Datasets by means of a Reference Dataset, Eliminating Ground Control Point Indication. International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences - ISPRS Archives; 2019; Vol. XLII-5/W2; pp. 85 - 91 Publisher: ISPRS online








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